High Temperature-Induced Spindle Destabilization Results in Aborted Pollen Production in Populus

High temperature can induce the production of 2n gametes and aborted pollen during microsporogenesis in Populus canescens. However, mechanism by which high induces abortion remains unknown. Here, was induced exposing male flower buds P. canescens to 38 41 °C; morphology, meiotic abnormalities, defects microtubular cytoskeleton, tapetum development were characterized. We found that dominant stage, temperature, duration treatment significantly affected percentage temperature-induced pollen. Damaged spindle microtubules depolymerized cytoskeletons observed, resulted many lagging chromosomes at anaphase I II as well aneuploid micronuclei, thereby generating grains. Tapetum disintegration also delayed. The anther dehisced normally, some viable grains released. no sufficient evidence showed delayed degradation responsible for abortion.